The Rejuvenation of Aunt Mary is a lost 1927 American silent comedy film starring veteran actress May Robson and released by Cecil B. DeMille's Producers Distributing Corporation (PDC).

Robson had first appeared in the Broadway play version of this story in 1907 when she was 49. In the film she returns to enact the same part 20 years later at the age of 69. A previous 1914 film version of the play had been produced minus May Robson.